ISOLATED PERFUSED RAT LIVER  

Commonly, isolated liver is prepared for perfusion by the method of Brauer (2) with minor modifications. Briefly, the rat is anesthetized and bile duct and portal vein are cannulated. The liver was transferred into the perfusion chamber, placed in a cabinet maintained at 37 ± 1 C° 

The perfusion fluid (120 ml) consisted of Krebs-Ringer bicarbonate buffer containing 5% bovine serum albumin and rat blood with a final hematocrit of 12.5% equilibrated with carbogen (95%O2 and 5% CO2). The flow of the recirculating medium is maintained in the range of 5.0 ± 1.5 ml.min-1.g-1, about corresponding to 3 times the hepatic flow rate in vivo. Infusion of sodium taurocholate solution (3 mM) is carried out at a 0.1ml.min-1 flow rate to compensate the bile acids normally present in the entero-hepatic circulation.

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Perfusion chamber,

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