ASSESSMENT

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ASSESSMENT OF INTESTINAL DRUG PERMEABILITY IN VITRO USING THE PERFUSED INTESTINAL SEGMENT MODELS.  

Pierre Gires ¹, Pr. John Woodley¹, Olivier Lacombe¹ & Pr Georges Houin ¹ 

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Inspired by the everted sac method, this model of a segment of intestine perfused ex vivo provides useful and complementary information to the sac system.  As shown on the pictures, an intestinal segment (duodenum, jejunum, or ileum) of around 10 cm is everted  and mounted in a chamber containing the test active substance and formulations dissolved in TC199 medium to mimic the mucosal compartment at 37° C (see accompanying diagram & pictures).  

The serosal side of the segment is continually perfused at a flow rate of 2 ml per minute.  The use of oxygenated TC199 on both sides of the segment ensures maximum viability for the duration of the experiment.  In addition to the advantages described above fo rthe sac, sampling at different time points means that a complete absorption kinetic is obtained on a single segment of intestine. Comparison of data obtained from the two techniques gives comparable permeabilities for test drugs.   

3  CONSULTANCY    

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